Editing Genetically Modified Organisms: BioEngineered aware

Grow More Foundation 25 Health Sciences Drive, Stony Brook NY 11790*Corresponding author email kmcreasey@growmorefoundation.org

Advancements in crop genomics and molecular biology are enabling us to combat plant pests, disease, environmental stresses as well as enhance nutrition and plant growth.

However, being at the forefront of agricultural biotechnology raises both societal questions and concerns, as well as the need for new regulatory oversight. It is imperative that scientists bridge the gap and enable an open, global dialogue so that all are informed.

Coming at the heels of the EU ruling to regulate genome edited (bioedited) crops in 20181, live births of bioedited human twins were reported in China2.

Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPER associated protein 9 (Cas9), components of the bacterial immune response3, to permanently alter the human genome thrusted the ethics of CRISPR to the front and center of public discourse.

Scientists and researchers across the globe expressed dismay at the premature application of bioediting in humans, recommending a moratorium on research, pending the development and adoption of clinical guidelines4. Though this was not the first time CRISPR made the headlines5. In 2016, the common white-button mushroom caused public outcry when regulatory bodies approved the non-regulated status for the bioedited non- browning fungi6.

To this end, regulatory bodies in America posted a request for comments, specifically on the safety of bioediting7, and held two public meetings on Agricultural Biotechnology Education and Outreach at the end of 2017. Concerned citizens voiced their fears, linking bioengineered crops with autism and cancer; farmers urged for more public outreach and education; and, non-government organizations (NGOs), such as Grow More Foundation, advocated for an independent, transparent and scientifically sound third-party review8. The heated discussions reflected the common widespread distrust of and misunderstandings about science and biotechnology.


About Grow More Foundation

Grow More is an NGO, 501(c)(3) non-profit established in 2017 comprised of scientists from across the globe developing globally accepted criteria for reviewing bioedited crops9. Their mission is to provide education, promote awareness and encourage transparency of biotechnologies in agriculture. Grow More does not accept funding from seed industry to remain non-conflict of interest and aims to encourage a global dialogue between scientists, society, industry and regulatory. They believe that the science, safety and environmental effects of each application of bioengineering or bioediting, must be independently and transparently evaluated.

This is not the first-time the ethics and safety of modifying an organism’s DNA has been questioned. For over two decades, the creation and consumption of bioengineered crops has been fraught with controversy.

Beginning with the transgenic better-tasting garden tomato in the mid 1990s (Box 2) to the most recent edited soybean with reduced trans fats10, fear, concern and uncertainty have negatively dominated the dialogue.

This attitude is perhaps not surprising, given the contrast in regulatory approval of modified crops for human consumption, with the US and EU representing opposite ends of the regulatory spectrum.

Approval of the CRISPR mushroom in the US set the precedent for non- regulated status of all subsequent CRISPR organisms that do not contain any foreign DNA and were created by precisely targeting and editing the native DNA.

Meanwhile, the EU ruled that CRISPR-mediated genome editing is essentially equivalent conventional bioengineering methods and therefore should be regulated. Therefore, bioedited crops cannot be created, tested or imported within the EU without prior regulatory approval, greatly impacting science, and pushing many scientists to act11,12.

Regulatory bodies in Nigeria recently approved bioengineered Bacillus thuringiensis (Bt) resistant cowpea13, in contrast with unsubstantiated claims that farming Bt cotton in Bangladesh correlated with farmer suicide rates14, reducing farmers to illegally growing Bt Brinjal (Richard Roberts, Pers. Comm.).

Bioengineered plantain field trials were recently successful in Uganda15, whereas several years prior, bioengineered rice field trials in the Philippines were destroyed by activists16. Though many EU countries ban the cultivation and import of bioengineered crops for human consumption, importation of bioengineered corn and soy for livestock feed is permitted17.

Everything we eat today has been selectively bred for favourable (genetic) traits and domesticated, and thus, technically, these crops are genetically modified. Bioediting could be viewed as one of many tools or technological advances that can be utilized to help us towards food security.


You say modified, I say edited

The first bioengineered crop on the market was tomato, and thirty years on it may well be the first bioedited crop also. Studies highlight the potential applications of bioediting in tomato fruit ripening18 and added flavour19. There is variation in size, shape and colour of tomatoes, due to domestication and selective breeding that modified ‘natural’ wild tomatoes, giving us ‘natural GMO’ tomatoes. Thanks to advanced breeding practices, we have long-lasting and shelf-stable tomatoes. However, in breeding stronger, bigger tomatoes, flavour was negatively impacted. To overcome this, Flavr Savr20 was created in the 1990s by the US company Calgene. They were committed to an open dialogue with the public to build confidence and reassurance, voluntarily seeking government approval and labeling. Whilst in the EU, the UK company Zeneca bioengineered tomatoes using similar technology and manufactured purees that outsold other tomato-based products in UK supermarkets. However, this was only for a short time. Why is this? The media hysteria caused by the ‘Pusztai affair’21 involving rat feeding studies had lasting negative consequences on the perception and acceptance of biotechnology in the EU22. No bioengineered tomatoes have been commercially available since the 1990s, and yet ‘non-GMO’ labels can be found on tomatoes and tomato-based products in many countries.

Historically, farmers have always incorporated technological advances into their crop and livestock management: from prehistoric ploughs to labour-saving machinery during the Industrial Revolution, and on to the use of pesticides and cross-bred high-yielding crops during the Green Revolution of the late twentieth century23.

Since these advances, farming has become far removed from our everyday lives, with children being unable to identify and fearful of fresh produce that their processed food was derived from. Most people are unaware of the issues faced by growers and the role of breeders in agriculture.

As regulatory bodies around the world debate the regulation of bioedited fungi, plants, animals and humans, the dialogue is further clouded due to the lack of standardization.

Bioengineered crops are produced by the transfer of genetic material from an unrelated species (transgenic), or related (cisgenic), and are commonly referred to as genetically modified organisms (GMOs).

Marketing initiatives dictate the binary concept of GMO or non-GMO, providing an attractive simplified statement for consumers. Such categorization inaccurately portrays that there are many more bioengineered crops commercially available than in reality.

Furthermore, inorganic products (as opposed to organic, i.e., alive, not to be confused with the alternate definition of grown with ‘natural chemicals’) lack DNA to be bioengineered in the first place, e.g., Himalayan salt and water.

This misleading labelling does not enable consumers to make better decisions, but instead plays on their fears at their own expense, literally.

Exploitation of GMOs further detaches society from the science, impacting stakeholders and policymakers. Alike biotechnology, labelling should adhere to ethical standards, and the misuse should be prevented by law24. At the end of 2018, American regulatory agencies announced the ruling for the mandatory labelling of bioengineered foods by 202025.

Yet, this ruling does not cover foods derived from bioedited crops. Unsurprisingly, consumer advocacy groups raised concerns that not labelling would be just as misleading as labelling water non- GMO, or add greater meaning to the alternative of non-GMO. Standardized food labelling would prevent such fear-based marketing and enable all to make informed choices.

So, why is bioengineering so controversial? The difference that sets biotechnology apart from other accepted methods for developing crops and animals is that it provides much greater control and precision of the genetic changes introduced. This stands in stark contrast to other accepted traditional bioengineering techniques to randomly create genetic changes, used in the first generation of bioengineered crops, namely radiation and chemical mutagenesis.

Science evolves quickly, and it is not surprising that when feeling left behind by advances, laypersons turn to sources that are easily digestible, often ignoring the credibility of the citations or the conflicts of interest, and too often conclude that correlation equates with causation.

Thus, “carcinogens mutate our DNA and cause cancer” becomes “genetic modification of our food causes cancer”. Even the discredited and retracted study on the association of herbicide- resistant corn and cancer in rats26 continues to be referenced and disseminated by GMO opponents as scientific proof of the alleged carcinogenic properties of GMOs.

With such established fear and distrust of the most commonly cited and controversial application of bioengineering, i.e., herbicide tolerance, how can we enable a new narrative?

Most can agree on the need to achieve food security, safety and sustainability. We can also agree that there will be insufficient arable land and fresh water to feed the growing world’s population. And, that unpredictable weather changes will directly impact and impede harvest.

Bioengineering has already saved papaya from viral infection, biofortified rice and plantain, and holds the potential to save cocoa from fungal infection, cassava from viral disease, maize from Fall armyworm, and coffee from climate change.

Science is about truth, and truth without transparency is meaningless. Without understanding the empiricism and scientific rigour of peer-review, as well as the testing of the scientific process, misinformation will continue to flourish, sow discord, and breed fear. 

Only if everyone is accurately informed, a formulated coherent, inclusive and productive dialogue can ensue. And, considering that public funding is a major contributor to research, scientists owe it to society to communicate widely. Just as Technology, Entertainment, Design (TED) conference talks27 are viewed by millions and voluntarily translated into more than a hundred languages, qualified experts could lead such communications efforts.

Regardless of stance, it is crucial for scientists, farmers, regulatory bodies, stakeholders, manufacturers, and most importantly consumers, from developed as well as developing countries, to come to the same table and put personal beliefs aside to devise a global and substantive policy on the creation and consumption of bioedited foods.


Terms that are used to describe molecular methods and application to agriculture are briefly explained.

Bioedited Term covering a wide range of molecular methods that are newer and more precisely modify the genome of an organism, such as CRISPR/Cas9 biotechnology (see definition below); and is used interchangeably with genome edited.

Bioengineered Alternative term for genetically engineered or genetically modified; a bioengineered organism contains DNA that was inserted into its genome that originates from a different organism. The DNA encoding the trait could come from the same species or a completely different one; for example, a gene from a bacterial species was introduced into soybeans, corn, and several other crops to make them more resistant to insect attacks.

Cas9 Bacterial protein that is guided to its target by a short sequence that matches and then cuts DNA and originated as a component of bacterial immune systems.

Cisgenic Type of bioengineering modification where the genetic trait introduced into the organism was from the same or a closely related species or wild relative. In theory the trait could also have been introduced into the organism via conventional crop development methods such as breeding.

CRISPR Technology derived from clustered, regularly interspaced, short palindromic repeats (CRISPR). This acronym originally referred to one aspect of the bacterial immune response to viruses, specifically, the component in the bacterial genome that contains small fragments of viral DNA. These fragments serve as guides for the Cas9 enzyme to identify invading viral DNA in order to cleave it and stop the virus from replicating in the cell. Currently, CRISPR and/or CRISPR/Cas9 is used as a general term to refer to the technique or method of bioengineering that forces this system to modify a wide variety of organisms.

GMO Abbreviation of genetically modified organism; a general term referring to any bioengineered organism.

Transgenic Type of bioengineering modification where the DNA modification introduced into a species originated from a different species that is not sexually compatible with the recipient organism. Bioengineered soybeans that are resistant to glyphosate are an example of a transgenic plant crop.


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The authors thank Denis Murphy for his feedback and contributions to this article. No competing interests declared.

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